Other

Part:BBa_K2082251:Design

Designed by: Pascal Schmidt   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-14)


Sequence for rpoZ Knock-Out in E. coli through CRISPR/Cas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 596
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1808
    Illegal SapI.rc site found at 2565


Design Notes

Coming soon


Source

Coming soon

References